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Sunday, August 9, 2020 | History

2 edition of Canine adenovirus type I: a potential viral expression vector for the rabies glycoprotein gene? found in the catalog.

Canine adenovirus type I: a potential viral expression vector for the rabies glycoprotein gene?

Serge Sira

Canine adenovirus type I: a potential viral expression vector for the rabies glycoprotein gene?

by Serge Sira

  • 215 Want to read
  • 12 Currently reading

Published .
Written in English


The Physical Object
Pagination108 leaves
Number of Pages108
ID Numbers
Open LibraryOL18049148M

Study Infectious V flashcards from Caroline O. on StudyBlue. o Flea feeds on infected host -> bact blocks flea provent. And contaminates feed site -> some bac killed at site -> survive in macrophages -> secrete anti phagocytic and antiinflam proteins -> EC survival via siderophores and capsule. In , most were types 2, 3 and 7. In , most were types 2, 3 and 1. Adenovirus type 7 infection rates peak during May to September, with 55 percent of patients in the age group 0 to 4 years. An nationwide outbreak of Adenovirus 7 infection (75 cases) was reported in

Canine vector-borne infections have gained importance in Germany due to growing tourist traffic and an increased import of dogs from abroad. Endemic regions for pathogens such as Leishmania infantum, Hepatozoon canis, Ehrlichia canis, Anaplasma platys and Dirofilaria spp. are the Mediterranean area and southeastern Europe. DNA vaccines are the third generation vaccines based on purified plasmid preparations containing transgenes that encode antigenic/therapeutic proteins or peptides capable of triggering an immune response against a wide range of diseases. This vaccine platform presents several attributes that confer distinct advantages over other vaccine technologies in terms of safety, ease of fabrication and Cited by:

Canarypox virus has been an effective vector in vaccines for canine distemper, rabies, and feline leukemia. ,,, For instance, a canine canarypox virus–vectored distemper vaccine is commercially available worldwide for administration by injection (Recombitek CDV). Fenner's Veterinary Virology 4E is the long awaited new edition of Veterinary Virology 3E which was published in Fully revised and updated by the new author team, Part I presents the fundamental principles of virology related to animal infection and disease and Part II addresses the clinical features, pathogenesis, diagnosis, epidemiology and prevention of individual diseases.


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Canine adenovirus type I: a potential viral expression vector for the rabies glycoprotein gene? by Serge Sira Download PDF EPUB FB2

E1-deleted adenovirus vectors based on simian serotypes also expressing the rabies virus glycoprotein are under development for human immunization. 33 These vectors are highly immunogenic and induce protective titers of rabies virus–neutralizing antibodies in nonhuman primates after a single low dose (10 9 vp) given intramuscularly before.

Recombinant adenoviral vectors for expression of cellular sensors are engineered (Fig. 1) by replacing the E1 gene of the viral genome (Bett et al., ; Hitt et al., ) with cDNA encoding the gene of interest fused to a fluorescent protein or with a reporter gene l of the E1 gene renders the adenoviral vectors replication deficient; however, they can be propagated by.

Adenoviruses are one of the most genetically diverse DNA viruses and cause non-life-threatening infections in the ocular, respiratory, or gastrointestinal epithelium of a diverse range of hosts.

Adenoviruses are excellent vectors for delivering genes or vaccine antigens to the target host tissues and are being tested in several vaccine and gene therapy studies. Adenovirus-based vectors offer Author: Shakti Singh, Rakesh Kumar, Babita Agrawal. Other potential uses include vaccines with overexpression of viral proteins such as rabies virus glycoprotein or cancer therapy This pathophysiology is the reason CAV-2 vectors are.

Because canine adenovirus 2 (CAV2) is licensed for use as a live vaccine for dogs and has an excellent efficacy and safety record, we used this virus as an expression vector for the RVG.

The recombinant CAV2-RV G produces virus titers similar to those produced by wild-type CAV2, indicating that the RVG gene does not affect virus replication. Additionally, E1-deleted and replication-competent recombinant canine type 2 adenovirus expressing the rabies glycoprotein was constructed, and it prevented both canine adenovirus type 2 infection.

Suggested Citation:"Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety." National Academy of Sciences. (NAS Colloquium) Genetic Engineering of Viruses and Viral Vectors.

Washington, DC: The National Academies Press. doi: / ×. Monosynaptic rabies tracing of inputs to Cre-expressing starter cells.

Monosynaptic tracing with G-deleted rabies (RVdG) involves expression of the EnvA receptor, TVA, and rabies glycoprotein (G) in starter cells are subsequently infected specifically with EnvA + RVdG, which is trans-complemented by G to produce G + RVdG that spreads trans-synaptically to neurons Cited by: Expression of the HSP70 C-terminal gene linked to the hantavirus glycoprotein Gn can augment cellular and humoral immunity and protects mice from a virulent challenge.

Co-expression of HSP70 and PRRSV gp3 and gp5 glycoproteins in an RDAd vector also enhances immunity to the antigens and improves vaccine efficacy [ 83 ].Author: José M. Rojas, Noemí Sevilla, Verónica Martín. @article{osti_, title = {A replication-deficient rabies virus vaccine expressing Ebola virus glycoprotein is highly attenuated for neurovirulence}, author = {Papaneri, Amy B.

and Wirblich, Christoph and Cann, Jennifer A. and Cooper, Kurt and Jahrling, Peter B. and Integrated Research Facility, National Institute of Allergy and. The history of heterologous gene expression in adenovirus (Ad) goes back to the discovery of simian virus 40 (SV40) contamination in the s of inactivated Ad strains 1–5 and 7 vaccines during Cited by: Although vaccines are available, rabies still claims more t human lives each year.

In most cases, rabies vaccines are given to humans after their exposure to a rabid animal; pre-exposure vaccination is largely reserved for humans at high risk for contacts with the virus.

Most cases of human rabies are transmitted by dogs. Dog rabies control by mass canine vaccination campaigns Cited by: 2. Applications of viral vectors have found an encouraging new beginning in gene therapy in recent years.

Significant improvements in vector engineering, delivery, and safety have placed viral vector-based therapy at the forefront of modern medicine. Viral vectors have been employed for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic, and Cited by:   The very first article to describe optogenetics in neural systems used viruses as delivery vectors (Boyden et al., Nat Neurosci 8(9)–, ).

Cited by: 1. A vaccinia recombinant expressing the rabies virus glycoprotein was an early example of a successful pox virus vector useful in immunization ().The vector was constructed by the insertion of the encoding cDNA for the rabies virus glycoprotein in the thymidine kinase locus of the Copenhagen strain of.

A recombinant anti-rabies vaccine (VRG, Raboral) has been made by inserting the gene for the surface glycoprotein of rabies into vaccinia virus, the virus used in smallpox vaccinations. The recombined virus appears safe for humans but is used for treating wild animals since (because it.

Recombinant protein vaccines. Most of the vaccines under investigation today are based on highly purified recombinant proteins or subunits of pathogens ().The classical example of recombinant protein vaccines currently in use in humans is the vaccine against hepatitis B (Table 1) ().Hepatitis B virus (HBV) infection is a chronic liver disease occurring by: A vaccine is a biological preparation that provides active acquired immunity to a particular disease.A vaccine typically contains an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe, its toxins, or one of its surface : D   There are two major types of vector systems for delivery of recombinant DNA (rDNA) to human tissues: nonviral and viral (Luo et al.

).Viral vectors provide the highest gene transfer and transgene expression efficiencies in vivo, which is the main impetus for the use of virus-based vectors in ~75 % of reported rDNA-based clinical protocols (Luo et al.

).Cited by: 1. The Rhabdoviridae are classified as a group based on a similar conical or bullet-shaped appearance by electron microscopy.

The laboratory diagnosis of rabies is most often performed for the postmortem examination of animals that have potentially exposed a human to the disease by a bite or other transdermal contact with saliva or neural tissue. • If viral genes are deleted then recombinant vector can be propagated in helper cell lines only • Most vectors derived from the adenoviral genome are replication deficient • Adenovirus can be used as vector for transient expression – This virus bearing foreign DNA can be used to produce the foreign protein in many different cell types.“The duration of immunity for canine distemper virus, canine parvovirus, canine adenovirus type 1 and 2 and the leptospiral components are at least 12 months.

However, the duration of immunity for canine parainfluenzavirus has not been determined.—Re-vaccination Scheme:A single dose of Vanguard 7 to be given annually thereafter”.(Italics.Plant-based vaccine technologies involve the integration of the desired genes encoding the antigen protein for specific disease into the genome of plant tissues by various methods.

Agrobacterium -mediated gene transfer and transformation via genetically modified plant virus are the common methods that have been used to produce effective by: